Gukata-Kwerekana Ishusho Ifungura Amabanga ya Nuclear Pore Molecular Igenzura ryumuhanda

Kimwe nabategura imijyi bategura neza ibinyabiziga bigenda mumujyi rwagati, selile ziyobora neza kugenda kwa molekile kumipaka ya kirimbuzi. Gukora amarembo ya microscopique, inganda za pore nucleaux (NPCs) zashyizwe mubice bya kirimbuzi bikomeza kugenzura neza ubwo bucuruzi bwa molekile. Ibikorwa byubaka biva muri Texas A&M Ubuzima birerekana uburyo buhanitse bwo guhitamo ubu buryo, bushobora gutanga ibitekerezo bishya kubyerekeye indwara zifata ubwonko ndetse niterambere rya kanseri.

Gukurikirana Impinduramatwara Inzira ya Molecular

Itsinda ry’ubushakashatsi bwa Dr. Siegfried Musser muri Texas A&M College of Medicine ryakoze iperereza ku iperereza ryihuse ryihuse rya molekile binyuze mu mbogamizi ya nucleus. Ikirangantego cyibidukikije cyasohoye ibisobanuro birambuye kubyavuye mu mpinduramatwara byashobotse hakoreshejwe ikoranabuhanga rya MINFLUX - uburyo bugezweho bwo gufata amashusho bushobora gufata mikorobe ya 3D iboneka muri milisegonda ku munzani inshuro 100.000 nziza kuruta ubugari bw'umusatsi w'umuntu. Bitandukanye nibitekerezo byabanje kubyerekeranye n'inzira zitandukanijwe, ubushakashatsi bwabo bwerekana ko inzira za kirimbuzi n’ibicuruzwa byoherezwa mu mahanga bisangiye inzira zuzuzanya mu miterere ya NPC.

Ubuvumbuzi butangaje Ikibazo kiriho

Ubushakashatsi bwakozwe nitsinda bwerekanye uburyo butunguranye bwumuhanda: molekile zigenda zinyura mumihanda ifunze, zigenda zuzuzanya aho gukurikira inzira zabigenewe. Igitangaje ni uko utwo duce twibanda hafi yurukuta rwumuyoboro, ugasigara hagati rwagati, mugihe iterambere ryabo ritinda cyane - inshuro zigera ku 1.000 gahoro gahoro kuruta kugenda nta nkomyi - kubera imiyoboro ya poroteyine ibangamira ibidukikije.

Musser asobanura ko ibyo ari “ibintu bigoye cyane mu mihanda yatekerezwa - inzira ebyiri zinyura mu bice bigufi.” Yiyemerera ati: “Ibyavuye mu bushakashatsi byerekana guhuza ibintu bitari byitezwe, byerekana ko bigoye kuruta ibyo twatekereje mbere.”

Gukora neza Nubwo Inzitizi

Igishimishije, sisitemu yo gutwara NPC yerekana imikorere idasanzwe nubwo izo mbogamizi. Musser atekereza ati: "Ubwinshi bwa NPC bushobora gukumira imikorere y’ubushobozi buke, bikagabanya neza kwivanga mu ipiganwa ndetse n’ingaruka zo gukumira." Ibiranga imiterere yihariye igaragara kugirango irinde molekulari gridlock, Hano'sa yongeye kwandikwa hamwe na syntax itandukanye, imiterere, nigika cyavunitse mugihe ubitse ibisobanuro byumwimerere:

Imodoka ya molekulari ifata inzira: NPCs Yerekana Inzira Zihishe

Aho gutembera neza muri NPC's hagati ya axis, molekules zisa nkizinyura mumuyoboro munani wihariye wo gutwara abantu, buriwese igarukira kumiterere isa nkivugwa kuri pore.'Impeta yo hanze. Iyi gahunda yerekana uburyo bwububiko bufasha kugenzura imigendekere ya molekile.

Musser asobanura,“Mugihe umusemburo wa kirimbuzi uzwiho kuba urimo a'icomeka hagati,'ibihimbano nyabyo bikomeje kuba amayobera. Mu ngirabuzimafatizo z'umuntu, iyi ngingo irahari't byagaragaye, ariko kugabana imikorere birashoboka-na pore's ikigo gishobora kuba inzira nyamukuru yohereza hanze ya mRNA.”

Guhuza Indwara n'imbogamizi zo kuvura

Imikorere mibi muri NPC-irembo rikomeye-yahujwe n'indwara zikomeye zo mu mutwe, harimo ALS (Lou Gehrig'indwara), Alzheimer's, na Huntington'Indwara. Byongeye kandi, ibikorwa byinshi byo gucuruza NPC bifitanye isano no gutera kanseri. Nubwo kwibasira uturere tumwe na tumwe bishobora gufasha mu buryo budasubirwaho guhagarika inzitizi cyangwa gutwara umuvuduko ukabije, Musser arihanangiriza ko guhindura imikorere ya NPC bitera ingaruka, bitewe n’uruhare rw’ibanze mu mibereho y’akagari.

“Tugomba gutandukanya inenge zijyanye nubwikorezi nibibazo bifitanye isano na NPC's guterana cyangwa gusenya,”yanditse.“Mugihe indwara nyinshi zishobora kuba ziri mubyiciro byanyuma, ibidasanzwe birahari-nka c9orf72 ihindagurika rya gene muri ALS, ikora igiteranyo kibangamira umubiri.”

Icyerekezo kizaza: Gushushanya inzira zumuzigo hamwe na Live-Cell Imaging

Musser n'umufatanyabikorwa Dr. Abhishek Sau, wo muri Texas A&M's Laboratwari ihuriweho na mikorosikopi, teganya gukora iperereza niba ubwoko butandukanye bwimizigo-nka ribosomal subunits na mRNA-kurikira inzira zidasanzwe cyangwa guhurira munzira zisangiwe. Imirimo yabo ikomeje hamwe nabafatanyabikorwa b’Ubudage (EMBL na Abberior Instruments) irashobora kandi guhuza MINFLUX kugirango ifate amashusho nyayo mu ngirabuzimafatizo nzima, itanga ibitekerezo bitigeze bibaho ku bijyanye n’ingufu zo gutwara ibitwaro bya kirimbuzi.

Dushyigikiwe ninkunga ya NIH, ubu bushakashatsi bwongeye guhindura imyumvire yacu yibikoresho bya selile, byerekana uburyo NPC ikomeza gahunda muri metero nini ya microscopique ya nucleus.